dna modification (Thermo Fisher)

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Thermo Fisher dna modification
Dna Modification, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modifications/DNA/pmc12857406-83-7-12
Average 99 stars, based on 1 article reviews

dna modification - by Bioz Stars, 2026-09

99/100 stars

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Article Title: Supporting Information Global Profiling and Inhibition of Protein Lipidation in Vector and Host Stages of the Sleeping Sickness Parasite Trypanosoma brucei
Article Snippet: Affinity enrichment were performed for all samples as before with the following modifications: NeutrAvidin agarose resin (Thermo Scientific, pre-washed 3 × 0.2 % SDS in PBS; typically 50 μL of bead slurry was used for 0.5 mg of lysate) was used in place of the magnetic Dynabeads.

Incubation:

Article Title: An Acid‐Oxidising Solution Containing Hypochlorous Acid Reduces Staphylococcus aureus and Improves Bacterial Diversity in Epidermolysis Bullosa Wounds
Article Snippet: .. Genomic DNA was extracted using a Maxwell 16 LEV Blood DNA Kit (Promega, Madison, WI) implemented on a Maxwell 16 Instrument, following the manufacturer's instructions with minor modifications: lysozyme incubation (10 ng/μl lysozyme; Thermo Fisher Scientific, Waltham, MA) for 30 min at 37°C and bead beating (5 min at 30 Hz twice, 2 min rest in between) using a TissueLyser III (Qiagen, Germantown, MD). ..

Protein Extraction:

Article Title: Proteomic Profiling Unraveling the Role of Lactate Dehydrogenase a in Vascular Repair and Functional Recovery after Spinal Cord Injury.
Article Snippet: Spinal cord injury (SCI) leads to complex pathological cascades, including endothelial cell dysfunction and vascular degeneration.. In this study, we employed label-free quantitative proteomics to profile spinal cord tissue following injury, and identify altered molecular pathways.. Proteomic analysis identified lactate dehydrogenase A (LDHA) as significantly upregulated at day post-injury 7 (DPI-7) and a potential regulator of vascular endothelial growth factor (VEGF)-VEGFR2 signaling.

Lysis:

Western Blot:

Article Title: DNA methylation insulates genic regions from CTCF loops near nuclear speckles
Article Snippet: The protein concentration of the lysates was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific, 23225). .. Immunoblotting was performed according to standard procedures with the following modifications: a 3–8% Tris acetate gel (Thermo Fisher Scientific, EA03752) was used, and samples were run for 2 hr 15 min at 80 V at 4°C in 1 X Tris-acetate running buffer (Thermo Fisher Scientific, LA0041) except for the loading control which was run for 90 min. .. Transfer was conducted in 1X transfer buffer (Research Product International, 501977679) containing 10% methanol and 0.05% sodium dodecyl sulfate (SDS) onto an activated 0.45 μm PVDF membrane (Millipore Sigma, IPVH09120) for 2 hr at 15 V except for loading control which was transferred in 1X transfer buffer with 20% methanol at 12 V for 90 min.

Control:

Amplification:

Article Title: High-risk alcohol consumption is associated with mitochondrial damage-associated molecular patterns in people living with HIV
Article Snippet: Briefly, cell-free mitochondrial DNA was extracted from 100 μl of serum using a MagMaxTM Cell-Free DNA Isolation Kit (Applied Biosystems, USA) following the manufacturer’s instructions with minor adaptations (2.5 μL of the MagMaxTM Cell-Free DNA Magnetic Beads per sample was used instead of 5 μL per sample). .. Cell-Free DNA was eluted in 20 μl volumes and aliquots were stored at −80 °C. mDAMPs were assessed via amplification of sequences within the NADH dehydrogenase subunit 1 (ND1) and NADH dehydrogenase subunit 6 (ND6) regions of the mitochondrial DNA by real-time polymerase chain reaction as previously described with minor modifications using a StepOne Plus Real-Time Polymerase Chain Reaction system (Thermo Fisher Scientific, USA) ( , ). ..

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